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KMID : 0545120110210101043
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 10 p.1043 ~ p.1048
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Isolation and Identification of Antitumor Promoters from the Seeds of Cassia tora
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Park Yeung-Beom
Kim Seon-Bong
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Abstract
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A methanol extract of Cassia tora seeds was successively partitioned with diethyl ether, chloroform, ethyl acetate, and water, and the antitumor-promoting activity of the solvent fractions was determined by inhibition of Epstein- Barr virus early antigen (EBV-EA) activation induced by teleocidin B-4 in Raji cells. The diethyl ether (68.7%) and chloroform (91.2%) fractions and the hydrolysate (94.3%) of the ethyl acetate fraction had strong inhibitory activities. The chloroform and ethyl acetate fractions were chromatographed on silica gel and further purified by HPLC. Three active compounds, obtusifolin-2-glucoside (75.0%), chryso-obtusin-6-glucoside (56.8%), and norrubrofusarin- 6-glucoside (39.4%), were obtained from the ethyl acetate fraction, and two active compounds, questin (97.9%) and chryso-obtusin (53.8%), were isolated from the chloroform fraction.
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KEYWORD
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Epstein-Barr virus, antitumor promoter, Cassia tora, anthraquinone
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